Can I use psPAX2 and pMD2.G to package lentivirus with pCDF1-MCS2-EF1-copGFP OR pMSCV PIG?

Tianzi Wei @Tianzi-Wei

07 May 2019 1 10K Report

As described, this is my first time to use these plasmids for overexpressing the gene of my interests.

BTW:

the pCDF1-MCS2-EF1-copGFP is based on FIV not HIV, so I am not sure whether psPAX2 and pMD2.G could be used.

Thanks!

Chanuka Kulatunga

For the envelope, of course, you can do pseudotyping with pMD2.G.

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