Can I use PBS-TWEEN instead PBS in Histopaque 1077 to dilute blood for isolation of mononuclear cells?

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Can I mix TRITC-labeled dextran and FITC-labeled lectin for one injection instead of having to do two injections of each?

For my protocol, at end-point I have to do tail-vein injections of TRITC-labeled dextran (100 ml of 10 mg/ml) and FITC-labeled lectin (50 mg). However, the protocol I'm following states that they...

01 May 2024 8,090 1 View

HiPSCs-derived neuroprogenitors detaching from middle -not edges, of the well in P6 plates, thoughts?

Hi all, In my lab we are currently differentiating neurons from hiPSCs according to the protocol used in Satir, T et al, 2020 (doi: 10.1038/s41598-020-57516-7). We have been successful in the...

27 March 2024 7,900 0 View

HI Mark, Please: Do you think ¿Is there any correlation between the malaria pigment and the volutin granules?

HemInozoin cristals are very compact in human parasites. However in opther haemoparasites as order haemosporidia is observed volutin. Do you think is there any correlation between protein...

26 February 2024 3,806 2 View

Can freeze-drying of fermentable product alter the results of sugar profile of the sample?

We want to conduct the sugar profile (Arabinose, Xylose, Galactose etc...) of a fermentable feedstock from different varieties. To reduce the degradation chances of this fermentable feedstock we...

10 January 2024 1,548 3 View

Do DNA diffuse from agarose gels?

how fast do DNA and RNA fragments diffuse from an agarose gel?

15 August 2023 7,142 5 View

How to remove ethanol from carbon dots solution to obtain just carbon dots?

I am synthesizing carbon dots with ethanol as the solvent. I tried to do oven drying but they stick on the wall of the glass vial. I tried to do air drying also but still the same. I tried to do...

21 June 2023 3,838 4 View

Have someone found differences in the circadian profile of Sprague-Dawley rats from different vendors?

Hi :) I am about to start a project in the circadian field and I will need to decide from now on which vendor I am going to select to purchase my animals. I would like to ask you guys if somebody...

29 May 2023 1,441 0 View

Can we neutralize concentrated Nitric acid with 1 M Phosphate buffer?

I am trying to neutralize nitric acid since the pH of my solution drops to zero after nitric acid treatment of my sample.

18 May 2023 6,411 3 View

"Hydrodynamic Analysis of Flow and Reduction of Local Scour in Longitudinal Walls."¿?

Dear colleagues, consultants, and builders, I hope this message finds you well. I am reaching out to request your kind cooperation and support in disseminating the following survey. This survey is...

09 May 2023 7,674 0 View

Can somebody know about CAPN13 in cattle?

Hi i am looking about references of posible asociation of CAPN13 gene and meat quality in cattle if you have information i be grateful

01 May 2023 2,728 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View