Can i use overexpressed myc tagged hsp60 as a mitochondrial fraction control in western blot???

Madhuparna Chakraborty @Madhuparna-Chakraborty

05 October 2020 0 5K Report

I have cloned Yeast hsp60 with a myc tag in a plasmid with a gal inducible promoter in Yeast system. I have overexpressed this myc tagged hsp60 in all my samples. Can I use myc tag specific antibody to obtain a band of hsp60 as loading control bands in mitochondrial fractions from different samples??

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