Can I use NanoDrop?

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How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program?

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...

28 July 2024 9,165 2 View

What are some reliable methods to measure the concentration of H2 gas at ppb levels?

We'll have vials in which we expect H2 generation to occur. Over the first few days, the amount of generated H2 is expected to be at ppb level. GC (+ TCD) detector is best when your H2 level is...

18 June 2024 254 3 View

Hello.I have a starting material that has both carboxylic acid and alchohalic OH group.I added triphosgene to convert thoes groups to acid cholorid?

and chloroformate. But it takes 10 days to compete the reaction. How can I shorten the time of the reaction?

14 June 2024 2,924 3 View

How does the targeted adoption of emerging technologies contribute to the advancement of sustainable practices in e-commerce?

The integration of emerging technologies in e-commerce is not just a trend but a strategic approach to achieving sustainability. This encompasses a wide array of innovations, from advanced...

04 June 2024 8,135 2 View

In what ways does the selective integration of emerging technologies enhance customer satisfaction on direct-to-consumer (D2C) platforms?

In the rapidly evolving landscape of e-commerce, D2C platforms are increasingly leveraging emerging technologies to streamline their operations and provide exceptional customer experiences. The...

04 June 2024 5,231 0 View

In a simulated microgrid, by applying the flow reference to the flow controller, the performance of the system becomes unbalanced, what is the reason?

I have simulated a microgrid in Simulink MATLAB; DC link voltage is converted to AC voltage by PWM inverter; The control system is implemented using proportional-resonance or PR controllers and...

16 May 2024 8,479 1 View

How has the adoption of the Direct-to-Consumer (D2C) business model reshaped the dynamics and performance metrics within the e-commerce sector?

This inquiry seeks to explore the transformative effects of the Direct-to-Consumer (D2C) business model on the e-commerce industry. It aims to understand how D2C strategies have altered consumer...

24 March 2024 6,737 1 View

What are some reliable sources to obtain pure mineral specimens?

I need specimens of some minerals in order to carry out experiments aiming to decipher fluid-rock interactions at different compositions and PT conditions. The challenge is that mineral phases...

22 March 2024 6,347 0 View

Can advanced endoscopic rhinoplasty redefine nasal surgery with precision and minimal invasiveness, revolutionizing patient care?

"Could the ongoing advancements in endoscopic rhinoplasty techniques mark a transformative milestone in nasal reconstruction, offering unprecedented precision and minimal invasiveness? How might...

03 March 2024 1,023 1 View

What are the methods or blocks that exist to implement discrete-time proportional-resonance(PR) controller in Simulink MATLAB?

Hello In Simulink MATLAB, we have a three-phase microgrid that we want to improve its power quality with the help of a three-phase inverter and adding a proportional-resonance controller to the...

16 February 2024 1,975 2 View

Is there a method to measure the concentration of Cs, Sr, Co using spectrophotometer?

it's important to know that we are using the inactive elements of Cs, Sr, Co

25 July 2024 1,553 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Convergence of absorbency values to determine the total phenolic content of several solvents?

I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help

17 July 2024 5,151 5 View

Hi. Can the absorption measurements of a polymer in a solvent be considered reliable when using a spectrophotometer?

zzz

02 July 2024 918 2 View

Why does the antioxidant activity increase as the number of OH groups on the phenolic ring increases?

and Why do double bonds reduce it ?

13 June 2024 4,836 4 View

I want determine Iron by spectrophotometer? Anyone know about the spectrophotometric determination of iron and copper, kindly share the procedure?

spectrophotometric determination of iron and copper

04 June 2024 1,720 0 View

NanoDrop 3300 Error 8008?

Hi everyone, has anyone else experienced an 'Error 8008' with their NanoDrop 3300 (image attached), on a laptop which it previously worked fine on? I have tried uninstalling the software and...

02 June 2024 9,025 3 View

How to calculate the total phenolic contents (TPC) in plant extract sample?

How do I calculate the total polyphenol content in mg GAE/g. from the extract? Following is my data: Sample extract(stock): 0.01 gram in 1 ml Equation: y= 0.0078x - 0.0679, R2 =...

27 May 2024 1,911 2 View

How shall i consider extinction coefficient unit?

I want to determine the conjugated drug to IgG ratio by nanodrop. I know the extinction coefficient of IgG and drug by M-1cm-1 but all the unit in the nandrop program is based on g/ml and so on...

15 May 2024 9,097 4 View

Calculation of Phenolic content in herbal extract?

Please can you answer my question I have an extract that I dried and made different concentrations to calculate the percentage of phenolic compounds using Gallic acid as a standard using the Folin...

07 May 2024 5,617 3 View

Israel E. Ebhohimen

I suggest you use a conventional spectrophotometer.

Chinaza Godswill Awuchi

You may. Kindly check https://vetmed.illinois.edu/wp-content/uploads/sites/19/2018/05/NanoDropOneUserManual.pdf

Kindly take some lessons from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009954/

Donya Moradi

Thank you dear Chinaza Godswill Awuchi Israel E. Ebhohimen

Asif Ali

Use of conventional spectrophotometer would be better.

ScanDrop & NanoDrop are most common.

260/230/280 ratio show DNA, salt and protein concentration

For details of protocol.

https://youtu.be/vWiaLh0PjRE