Can cell lines have different DNA content?

I am doing calibration curves using CyQUANT Direct Assay to 10 different pancreatic cancer cell lines. I've noticed that the fluorescence of some cell lines double at the same cell number. Is this...

03 February 2021 3,587 2 View

Can I incubate two secondary antibodies at the same time for western blot?

I want to know if I can use a goat-anti mouse IgM-HRP and a goat-anti mouse IgG-HRP to treat a western blot membrane at the same time. Thank you

26 September 2020 1,332 6 View

Cordial saludo, necesito retirar nombre de la imagen diagnostica de mamografia con Python com lo hago?

en la imagen diagnostica aparece el nombre de cada imagen CCD, CCI deseo borrar la imagen del nombre alguien me puede ayudar agradezco su ayuda , gracias

11 May 2020 9,205 2 View

What metaphors are explaining the COVID-19 pandemic and how do they impact on the approach that is being considered from public health?

Media discourses around the coronavirus pandemic tend towards metaphorical expressions such as the war against an invisible enemy, of the ecosystem balance so that the Earth returns to its...

31 March 2020 3,098 14 View

Help debugging an old Fortran program?

Could someone please help me debug the attached Fortran program? I have reduced the program to just a MAIN and a subroutine. The program reads a matrix and calculates its determinant. I cannot...

26 March 2020 7,108 2 View

State with plural ethical background?

Let A be a person with a consequentialist ethics and B with a deontological ethics. Is it possible that A and B agree on the structure of the State? If so, how would they reach an agreement?

02 December 2019 5,019 6 View

How can I insert initial imperfections on a non-linear analysis from a subset displacement summation of previously elastic buckling analysis on ANSYS?

Hi Everyone, I am working on the strength capacity of clamped-clamped cold formed steel columns with lipped channel sections on ANSYS. For this case I perform a non-linear analysis (ARCLEN), and...

14 May 2019 8,058 12 View

How can I garantee that the CO2 coming out from my biorreactor won't be dangerous in a closed laboratory?

I need to sparge CO2 for my biorreactor, but I'm afraid the excess CO2 coming out from it will build up inside the laboratory. Is there any solution to this problem that doesn't involve expensive...

19 July 2018 9,316 5 View

How does the concentration of PBS affect the Immunoflourescent staining?

I'm doing immunofluorescent staining of dopamine neurons in mice brains. I've seen various protocols using different concentrations of PB or PBS in their blocking buffer. I would like to know how...

17 June 2018 7,334 3 View

Could viruses be the creators and basic modifiers of life being a type of bridge between abiotic elements and living organisms?

I mean, viruses are not "live organism" because they need cells to can do any action according with current biology, but, humans are not nothing and cannot live without plants and abiotic...

13 April 2018 5,289 3 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View

I'm using AMOS for SEM--Why does my chi-square value change when I specify groups?

My study involves 2 groups (Black and Latinx participants, N=816). I've run my structural equation models with the data all together and will get a completely different chi-square when I specify...

22 February 2021 9,342 4 View

Are negative valued indicators allowed in SEM?

Hello, Using secondary and continuous data, i am trying to run a SEM with two latents. One indicator has both negative and positive values. Is it wrong? (I am gonna run the model in AMOS) Thx on...

14 February 2021 2,801 8 View

Moderation test of reflective construct in AMOS?

Hi dear researchers, I need your guidance on the moderation test of a reflective construct (i.e., Uncertainty avoidance with three items) with high and low UA. I run the moderation test with the...

08 February 2021 370 7 View

How to obtain confidence intervals in Amos?

Dear all, I would like to know your opinion on the following issue I am struggling with. I am estimating a multigroup model with several factors all correlated among them (they represent...

04 February 2021 1,381 4 View

AMOS - Question regarding correlated errors in CFA, what next?

Hi all, Hopefully this is just a quick question for those of you au fait with CFA in AMOS. I am brand new to this. I'm looking at model (small sample size, yes I know) with 5 latent variables....

26 January 2021 4,425 2 View

Is it possible to implement a Latent Profile Analysis with AMOS?

Could you kindly recommend me any useful totorials to learn how this statistical anaysis should be applied? Thanks

10 January 2021 3,633 2 View

Can we use control variables during Structural Equation Modeling with Amos?

We want to explore a mediating relationship employing SEM with Amos SPSS. Our independent variable is attitude toward wife abuse. mediator is exposure to male marital violence, and outcome...

07 January 2021 4,481 3 View