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Questions related from Kadir Aslan
I’m working with a recombinant P450 enzyme that I’ve directed to the periplasmic space using a signal peptide. Since I will use this enzyme for downstream applications, maintaining its activity is...
13 May 2025 6,229 3 View
I recently received a Rosetta strain from a university research group, along with some expression constructs. When I asked for chloramphenicol to maintain the pRARE plasmid carrying the rare tRNA...
01 May 2025 8,563 2 View
Hello everyone, I recently cloned EGFP into pET-28a(+) and transformed it into BL21(DE3) cells. However, despite trying multiple conditions, I am not observing any yellow-green color or...
10 February 2025 8,592 8 View
Hi everyone, I’m working with primers where the initial binding temperature is around 60-62°C, but with tails, it increases to 69-70°C. Should I use step-up PCR, or is adding DMSO more advisable?...
18 January 2025 8,369 2 View
I am planning to clone an enzyme using the pET28a vector. I would like to ask whether the N-terminal His-tag alone would be sufficient for efficient expression and purification, or if it might...
02 December 2024 8,826 6 View
In many cloning protocols, researchers introduce an intermediate cloning step using a vector like pGEM-T before transferring the insert into the final destination vector. Could you elaborate on...
12 August 2024 6,438 2 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
26 July 2024 5,227 3 View
Hello, I have a methanol extract that I have let stand for 3 days, three times. I have evaporated the methanol and, just to be sure, mixed it with water and lyophilized it. Before performing HPLC,...
21 May 2024 1,890 13 View
Hello, After synthesis curcumin metal complexes, how can I purificate molecules with 1:1 and 1:2 metal-curcumin stoichiometry, and is there any reference study on this matter? In the literature,...
05 April 2024 8,610 4 View
Hello, In the literature, there are some MS/MS results that include hypothetical proteins, which can be shorter than 40 amino acids. I can also find these when I search for an organism in the...
05 April 2024 5,153 2 View
Hello, I've recently started exploring molecular docking applications, and I'm still in the early stages. I'd like to ask Can I choose a ligand by giving the amino acid sequence and then do...
04 December 2023 3,123 3 View
Hello, I've recently started exploring molecular docking applications, and I'm still in the early stages.I'd like to ask which proteins should be considered when examining the antimicrobial...
04 December 2023 777 4 View
I have a few substances that resemble curcumin, and I need to conduct antimicrobial tests, especially MIC experiments in a 96-well plate, with these substances. However, these substances are...
03 September 2023 6,191 4 View
I am conducting research on plant-microbe interactions and require a large quantity of sterilized soil for my experiments. While autoclaving is impractical for such volumes, I have adopted a...
01 January 1970 4,746 4 View
