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I’m working with a recombinant P450 enzyme that I’ve directed to the periplasmic space using a signal peptide. Since I will use this enzyme for downstream applications, maintaining its activity is...
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Hi everyone, I’m working with primers where the initial binding temperature is around 60-62°C, but with tails, it increases to 69-70°C. Should I use step-up PCR, or is adding DMSO more advisable?...
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In many cloning protocols, researchers introduce an intermediate cloning step using a vector like pGEM-T before transferring the insert into the final destination vector. Could you elaborate on...
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Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
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Hello, I have a methanol extract that I have let stand for 3 days, three times. I have evaporated the methanol and, just to be sure, mixed it with water and lyophilized it. Before performing HPLC,...
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Hello, After synthesis curcumin metal complexes, how can I purificate molecules with 1:1 and 1:2 metal-curcumin stoichiometry, and is there any reference study on this matter? In the literature,...
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Hello, In the literature, there are some MS/MS results that include hypothetical proteins, which can be shorter than 40 amino acids. I can also find these when I search for an organism in the...
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Hello, I've recently started exploring molecular docking applications, and I'm still in the early stages. I'd like to ask Can I choose a ligand by giving the amino acid sequence and then do...
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Hello, I've recently started exploring molecular docking applications, and I'm still in the early stages.I'd like to ask which proteins should be considered when examining the antimicrobial...
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I have a few substances that resemble curcumin, and I need to conduct antimicrobial tests, especially MIC experiments in a 96-well plate, with these substances. However, these substances are...
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I am conducting research on plant-microbe interactions and require a large quantity of sterilized soil for my experiments. While autoclaving is impractical for such volumes, I have adopted a...
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