As we all know that In the environment soil, primer of nitrite reductase(nirk) has been designed ,but primer of nitric oxide reductase(P450nor) has not .So if I use the primer of nirk to amplify envrionment soil and the primer of cyp55(A1,A2,A3,A4,A5) to amplify fungal strains(isolate from the environment soil ) ,the result whether can explain the fungal denitrification in the environment soil?