Hi,eyeryone.In the process of fungal denitrification,nitrite reductase(nir) and nitric oxide reductase(P450nor) participate in it. And the nirk gene encodes the nitrite reductase,cyp55 gene encodes nitric oxide reductase.now I have find the primer of nirk for PCR of soil samples,but the question is that cyp55 is a super family,different strain has different primer of cyp55,for example, primer of Fusarium oxysporum's cyp55A1 gene has been fund，now my sample from the rice paddy soil ，so I want to find the universal primer target cyp55 gene,do you konw this primer?I hope anything you know could tell me.thanks very much.I need your help.
Hi again, sorry for not answering earlier, I was on vacation. The problem with these genes in fungi as their variability. There are some quite degenerate primers for cyp55 genes which can be considered as universal, but from my experiences they produce more than one band after PCR of pure cultures of N2O producing fungi and after sequencing, there is no homology with known cyp55 genes. Other cyp55 primers were designed just for one or a few species so they are not universal at all.
I think it is this reference* but I am not sure if you will be able to read it. I will try to find some other later today, ok?
*Zhang and Shoun 2008. Purification and Functional analysis of fungal nitric oxide reductase cytochrome P450nor. In Poole RK (ed): Methods in Enzymology, vol 437, Globins and other Nitric oxide-reactive proteins, part B. pp 117-134
Hi,Jiří!
I am so glad to receive your message.You can not image how happy I receive it. last time I thought that maybe I have bothered you. i
The paper you recommend me is so good that I want to read it right now .And these papers you give me before，I have all download them，and all of them are useful，and some of them I have print to read.
hope to keep in touch with you.
best regards.
huifang
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