Can i use DH5 alpha competent cell instead of XL1 Blue ?

More Irfan Hussain's questions See All

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

SSR primers are species specific or we can use it for any plant species ?

Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...

20 February 2021 315 4 View

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

18 February 2021 6,483 3 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

How to plot Random Forest model as raster prediction map in RStudio?

Output_raster

27 January 2021 7,974 3 View

What solvent is suitable for ferric oxide nanoparticles?

Please help me suggest the appropriate solvent for the ferric oxide nanoparticles for the possibility of exploring their use in cancer treatment .. Your suggestions are very important to me.

11 January 2021 5,321 4 View

What are the (extrinsic) factors that influence the citation rate of a research article?

Dear RG members, Excluding the (intrinsic) factors such as quality and content of an article, what are the extrinsic factors/predicors that influence the citation rate of a research article? Is...

07 January 2021 3,410 3 View

Do you have datasets for Covid-19 Chest X-ray and CT Lung labeled dataset?

I am working on COVID-19 detection and analysis challenge. Please, share me the labeled dataset. My relevant articles are : 1. COVID-19 Detection in Chest X-Ray Images using a New Channel Boosted...

02 January 2021 3,609 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Irfan Hussain

Thank you very much! Cornelia Virus

Chen Yu

Yes, you can use DH5-alpha competent cells instead of XL1-Blue for many common molecular biology applications, but there are some differences to be aware of. Both DH5-alpha and XL1-Blue are E. coli strains commonly used for cloning purposes, especially for plasmid amplification. Here are some considerations:

Efficiency: Both strains are generally high-efficiency in terms of transformation, which means they are good at taking up foreign DNA (like plasmids). However, the actual efficiency can vary depending on the preparation of competent cells and the specific application.

Blue/White Screening: Both DH5-alpha and XL1-Blue support blue/white screening for recombinant clones when using vectors with the lacZα gene. This is useful for identifying successful inserts in cloning experiments.

EndA1 Mutation: Both strains have the endA1 mutation, which leads to a reduction in endonuclease I activity. This is beneficial for producing high-quality plasmid DNA, as it reduces degradation of plasmid DNA during isolation.

RecA1 Mutation: Both strains are recA1, which means they have reduced recombination activity. This is advantageous for maintaining plasmid stability, especially for plasmids with repetitive sequences.

Other Genetic Features: XL1-Blue cells have additional features like the mcr and mrr restriction systems, which can affect the cloning of certain DNA sequences. DH5-alpha lacks these systems, which can be a benefit or a downside depending on your specific application.

Application Specifics: For most standard cloning applications, including routine subcloning and plasmid amplification, both strains are suitable. However, for certain specialized applications, the unique features of each strain might be more or less advantageous.

In summary, for most general cloning purposes, DH5-alpha can be a suitable alternative to XL1-Blue. However, for certain applications or specific DNA sequences, one strain may be preferable over the other due to their genetic differences. It's always good practice to consider the specific requirements of your experiment when choosing a bacterial strain.

l Take a look at this protocol list; it could assist in understanding and solving the problem.