Irfan Hussain

7 Questions 15 Answers 0 Followers

Questions related from Irfan Hussain

My construct for protein expression is not in frame with plasmid ATG?

I have attached Ndel and xho1 site with my construct. i have digested my construct and PET30a both with ndel and xho1. but after in silico analysis using snapgene i found that my construct is not...

18 January 2017 5,642 3 View

Can i use DH5 alpha competent cell instead of XL1 Blue ?

The stated growth strain for DR274 vector is XL1 blue. But currently i have DH5 alpha in my lab. Can i use it as growth strain for DR274 vector ?

06 August 2016 712 2 View

What can be used in place of Acetic Anhydride for acetylation during mRNA whole mount in situ In situ hybridization?

for decreasing background during mRNA whole mount in situ In situ hybridization acetylation with acetic anhydride (0.25%) in triethanolamine is required. Is there any alternative to Acetic...

01 April 2016 2,108 2 View

How long can I store RNA extract from dengue serum sample at -70 c?

The serum sample collected 25 days ago from dengue samples were store at -70 c. Now is there any possibility that RNA can be extracted from these samples. As I have used a viral RNA isolation kit...

07 December 2014 1,473 6 View

Termite DNA extraction protocol?

Can someone suggest me an easy protocol of DNA extraction from termites preserved in ethanol.

17 November 2014 8,637 4 View

Can chicken embryonated eggs be used to increase the dengue virus titre?

What are the chances of propagation of dengue virus in embryonated eggs?

06 November 2014 8,572 1 View

How long can we store the serum at -20 c collected from dengue patients ?

We have collected blood from the dengue patients in sst tube, separate the serum and store it at -20c. Now how long can we store these samples? Is there any risk of decreasing and degradation the...

01 November 2014 3,454 4 View