I am currently making bacmid and consequent baculovirus using pDEST8, pFastBac and 438a vectors and recently switched to DH10EmBacY cells instead of DH10Bac due to the added YFP signal to monitor transfection, etc.
I was wondering if there is any reason for me to not use either of the two competent cells interchangeably to make bacmid DNA?
No, DH10Bac and DH10EmBacY cells are not interchangeable. They are different strains of E. coli cells used for different purposes.
DH10Bac and DH10EmBacY cells are both used for the generation of recombinant baculoviruses for protein expression in insect cells. However, they have different characteristics and uses.
DH10Bac cells are used in the Bac-to-Bac system developed by Thermo Fisher Scientific. They are used to generate recombinant bacmids containing your gene of interest in a baculovirus genome. These bacmids can then be transfected into insect cells to produce recombinant baculoviruses.
On the other hand, DH10EmBacY cells are part of the EMBacY system, an alternative system for generating recombinant baculoviruses. This system also involves creating recombinant bacmids, but the process and the specific cells used differ from the Bac-to-Bac system.
In general, you should follow the protocol and guidelines provided for each specific system and use the recommended cells for that system. Interchanging the cells may not produce the desired results, as each system has its own optimized conditions and requirements.
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