Is re-labeling an antibody a bad idea?

I labeled an antibody with FITC a year ago, titrated it for flow and it gave good results. I aliquoted it and store it at -20C away from light, as the kit recommends. I also have it wrapped in...

07 August 2015 2,506 8 View

Any recommendations for PBMC culture followed by lymphocyte and monocyte separation?

I will be doing experiments involving culturing and stimulating PBMCs and harvesting them 24 hours later. Basically, they will only be in culture for a day. I am interested in a potential...

06 July 2015 2,664 5 View

Any suggestion on the seeding density for primary monocytes in a 96-well?

I am doing a one-day assay with primary monocytes and seeding them at 3*10^5/well, as per a colleague recommendation. However I see others recommend much lower, but they seem to have different...

05 June 2015 5,786 3 View

How do I stimulate cell cultures with beta-estradiol?

I have been doing cell culture experiments that involve treating the cells with estradiol (E2). There are many papers published that state these cells are responsive to estrogen. The problem is...

04 May 2015 9,167 6 View

Has Anyone had good results with difficult-to-transfect cell lines?

I am working on transfecting Ramos cells and currently using electroporation: the optimized protocol for the Amaxa Nucleofector system. The problem is I am not getting any luciferase readouts. I...

03 April 2015 4,040 4 View

Who can help me with using Haploview for SNP analysis?

I have 3 large files with case control data for various SNPs on my gene of interest. The files are in the format .bed, .bim, and .fam. Apparently Haploview only accepts .ped and .info files. Do I...

02 March 2015 2,694 9 View

If I have poor PCR efficiency, does this mean my data is unreliable garbage?

I plotted Ct vs log conc. of my standards and the slope of the curve is -1.79, giving me an efficiency of 262%.

03 April 2014 8,872 18 View

Is it better to perform cell staining for flow cytometry in HBSS, or HBSS with 2% FCS?

I've seen it done either way.

01 February 2014 2,338 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View