KEGG/KAAS alternative tool

I wanted to analyse metabolic pathways of an organism and hence wanted to retrieve the KO annotations. However, my organism is not available in the gene set. Should I use an organism closely...

05 June 2013 345 2 View

Finding out the sequence of the given amplicon

the primer sequence and hence can't go for sequencing? Is there a way to find out these unknown amplified

11 December 2012 9,702 7 View

EBV as biomarker in ageing study?

Why do these ageing studies measure epstein-barr virus antibody titres? What's the significance of testing Ab against EBV and not other normal flora?

10 November 2012 9,867 5 View

Does A260/A280 have the ratio value of 3?

A commercial viral DNA extraction kit was used for extraction of DNA from HSV virus. The kit is a RNA carrier molecule for efficient binding of nucleic acids to the column membrane. Two sets of...

08 September 2012 5,292 10 View

Is it necessary to work with force fields while doing some docking study of peptide with proteins?

I would like to work on designing a peptide that works at the interface of two protein that interact with each other thus inhibiting this interaction.

08 September 2012 7,857 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View