I know that we should use API 20 NE for the identification of pseudomonas aeruginosa, but I don't have API 20 NE strips in the lab.
Not is the best approach to confirm specie identification, however could be a first step for genera confirmation, because you'll can combine the oxidase test and sugar metabolism. Most strains of Pseudomonas aeruginosa produces pigment and fluorescent colonies and they are able to grow at 42ºC.
If you have decided that your bacterium is Pseudomonas aeruginosa then why to do any test? It is not good science to give name to any bacterium without identifying it properly. And for that you have to follow different tests like biochemical, and 16S rRNA gene sequencing to name a few. Then only you would be able to be certain about the bacterium.
thank you guys. Actually, i did Gram stain, and the it turned out that the bacterium was Gram negative rod, then i did an oxidase inorder to differentiate between members of enterobacteriaccea and non-enterobacteriaccea. I assumed that the next step would be API 20e. How can i defferentiate between members of gram negative non-enterobacteriaccea rods?
Thanks.
Richard, if you are not going for 16S rRNA gene sequencing then follow Bergey's Manual for specific tests.
You can apply boichemical tests as described in Bergeys manual, you can use API from bioMerieux or Remel from Oxoid. In routine practice in can be economic to know that 99.9 % percent of oxidase-positive Gram-negative rods can be identified reliably as Pseudomonas aeruginasa if they combine typical appearance with positive gelatinase activity. Typical appearance means strog beta-hemolysis and production of metalic pigment on blood agar or green pigment on MH agar. Gelatinase activity can be tested on agar with gelatine.
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