Richard Maroun

6 Questions 11 Answers 0 Followers

Questions related from Richard Maroun

Can I use API 20E to identify a bacterium most likely to be pseudomonas aeruginosa ?

I know that we should use API 20 NE for the identification of pseudomonas aeruginosa, but I don't have API 20 NE strips in the lab.

13 November 2013 8,549 5 View

How does one perform phage titration and determine phage titer ?

I have isolated 11 phages from sewage and have stock solutions of them. I want to determine the phage titers for each. How can I do this?

16 October 2013 7,009 1 View

Characterize coliphages based on sensitivity to temperature, chloroform, and pH - can anyone help?

I just isolated 17 phages from sewage e-coli and was wondering if anyone can provide me with a protocol on how to achieve these tests. First I am going to determine the phage titer of the 17...

03 October 2013 1,162 0 View

What is a conventional method for extracting protein content from phage and running them on a gel?

I have isolated and purified 17 phages from sewage and I want to characterize them based on protein content using SDS-PAGE. Are there any kits for this ?

27 September 2013 4,775 3 View

I want to characterize 17 phages obtained from sewage based on host range, sensitivity to chloroform, temperature and pH?

I have 17 phages from sewage and want to test them on 50 bacterial strains obtained from UTI (urinary tract infected) patients as a means of characterization based on host range, i also want to...

19 September 2013 1,306 2 View

Which plate should I choose from the pics below following a double agar overlay technique in order to prepare high titer phage stock?

Double agar overlay technique

16 September 2013 1,892 9 View