https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813050/

I thought this article will help you.

Probably not, as they might be quite different enzymes, and you'll be comparing your fungal source with humans/mammals. Do you have the peptide sequences and possibly structure data, so you can make some educated guesses?

For reliable and really useful data, you'll need some human amylase. Possibly the porcine variant is suitable, or use recombinant human enzyme.

If you should be able to establish the fungal amylase as model system for the human one, this might suit as a quick and cost efficient tool to monitor e.g the extraction and purification of your inhibitor.

Dear Mariam mohamed Mostafa

I recommend the widely used a-amylase from porcine pancreas from Sigma.

Please find a protocol in the attached paper.

With kind regards

Christophe

May be try