From a journal, the standard calibration curve was prepared from a concentration of 1 to 10g/L. In my experiment, the maximum expected concentration of the solution is 0.1 g/L. Would the concentration value that I get will be accurate if I am using the standard curve?
Please be careful, the ideas given by others have been useful. But if the reported linear concentration range is from 1-10 g/L, it is possible that the responses obtained between 0 and 1 g/L did not increase linearly with concentration. So if you are going to analyse a sample with a concentration outside the reported linear range, you need to verify that you can get a linear response for concentrations between 0 and 1 g/L. If that is not possible, your other choice will be to concentrate your sample by a factor of 10 or more to come within the reported 1-10 g/L range.
Dear Siti Aishah, Better you prepare a new calibration curve for lesser concentrations because you may get the results in milligram/L too,
it is not advisable to use the standard curve that you taken so far because it doesn't give you the exact concentration in your sample.
You need a new one calibration curve please see... Preparation of. Calibration Curves. A Guide to Best Practice online www.nmschembio.org.uk/dm_documents/ lgcvam2003032_xsjgl.pdf
Thank you for the answers. So, I should prepare a standard curve for the lower concentration. I just hope that the curve will have r^2>0.99.
Dear colleague, you need prepare a new calibration curve for lower concentration (and not use the one found in the journal to match it to your matrices) but you need first to verify the limit linearity of your calibration curve to know if we can do it for the lower concentration or not
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