We are using the resazurin dye assay, specifically its fluorescence assay, to measure the growth of our bacteria using a BMG Labtehc Fluostar microplate reader. We have so many samples that it cannot fit a single plate. If we want to compare growth of bacteria from different plates, could we use a single gain adjustment value?

How about if we want to compare growth of samples from different experiments (i.e. pH experiment done a day/week before the salinity experiment)? Is it good practice to have the gain adjustment value unchanged between experiments? Or should we add like some kind of standards (i.e. cells with known amount that will only be added for the resazurin assay for both experiments)?

Thanks for the input.