I want to test drugs on different cerebral organoid slices (300µm+). Therefore, I want to characterize the cell viability of an organoid slice beforehand. Afterwards, the organoid slices shall be treated with different drugs and the cell viability shall be measured again. With this, I hope to measure the cytotoxicity.
So my questions are following:
Can I use a MTS assay to characterize a tissue such as an organoid slice?
Can I wash the sample thoroughly to do a MTS assay again with the same slice, or is there any problem I am not aware of?
Because its a tissue and not a small layer of cells, will there be any problem with the amount of cells for the MTS assay? I know it is usually only used for a very small number of cells, like in a 96-well plate.
Is there any other kind of assay, which would be better suited?
Thanks in advance!