Dear Researcher,

It is possible. You can study the following article:

Article Chitosan-PVA-CNT nanofibers as electrically conductive scaff...

You could also use the resazurin assay to study the viability in organoids, which is a non-invasive method.

Dear David!

Please You also look at the article:

Article Organotypic Brain Slice Cultures of Adult Transgenic P301S M...

Vitality Assays

The MTT assay is based on the conversion of the yellow tetrazolium salt MTT to purple formazan crystals by metabolic active cells. MTT (stock solution 5 mg/mL in PBS, pH 7.4) was added to the cultivation media to a final concentration of 0.5 mg/mL. The slices were incubated for four hours under cultivation conditions, washed with PBS, and transferred into a mixture of SDS (10%, w/v, Roth), DMF (25%, v/v, Biosolve, Valkenswaard, Netherlands) and water to dissolve the formazan crystals. After 24 hours the samples were centrifuged and the absorbance of the supernatant (150 mL) was recorded at 562 nm and 690 nm. The metabolic activity was calculated as follows: (A562– A690)/slice volume. In both assays triton X-100 (1%) was used as a control for cell death after four hours of incubation. Each analysis was performed in duplicate with 3 to 4 slices per time point for the MTT assay and 5 to 6 wells (each containing 2 slices) per time point for the LDH assay.