Hello, everyone!
I'm currently facing a challenge in consistently extracting and estimating the concentration of chlorophyll a in the cyanobacteria I'm researching, mainly due to the high frequency and quantity of samples I'm dealing with, as well as due to the complexity of different extraction methods.
After conducting some experiments, I discovered that the method outlined in Azua-Bustos' paper (Article Gloeocapsopsis AAB1, an extremely desiccation-tolerant cyano...
) proves to be effective for my extractions. This method employs warm methanol as the solvent.While searching for equations to estimate chlorophyll concentration, I came across an article by Ritchie (Article Consistent Sets of Spectrophotometric Chlorophyll Equations ...
) that provides the necessary equations using methanol as the solvent. However, the extraction method in the Ritchie paper involves a mixture of acetone (90%), methanol and ethanol as solvents. The final absorbance measurement is taken in methanol, along with the associated equations.Now, my question is: Is it possible to combine the extraction method from the first paper with the equations from the second paper? Or does each extraction method require a specific set of equations, even if the absorbance measurements are both made in methanol?
Thanks a lot for the help! :)
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