I want to grow aerobic mycobacteria in 96 well plate without contamination for 3 days time. If I grow with the plastic cover on will it cut off their oxygen?
Hi all, I am using the RoB2 tool on Excel for the first time today, and have been faced with some issues. When filling in each domain, the algorithm has successfully calculated the domain risk...
28 January 2024 5,281 1 View
Where does this RV followed by a number come from?
16 August 2023 3,443 3 View
16 August 2023 374 3 View
I have run siRNA transfection on SNO cell lines. I seeded in a 6 well plate, incubated at 24 hours and treated with different volumes and concentrations of the siRNA and transfection reagent. I...
29 June 2023 5,439 1 View
I am a 3rd medical student and I'm interested in working on a meta-analysis/ systematic review, however, I am a fledgling when it comes to this realm of research. I have published an LTE and I'm...
11 May 2023 5,860 4 View
Hi, Do you have recommendations of any articles on EE in shrimp / crustaceans. If there are any published studies out there it will be limited. Unfortunately all the info I have come across...
05 February 2023 562 5 View
Hi, I am looking for papers on electrical stunning in crustaceans where the pros and cons of dry versus wet stunning is discussed / described. Thanks.
07 December 2022 117 3 View
What information do the blue(positive contour) and green(negative contour) tell me in HSQC? Can I discover any information about a structure depending on if the contour is negative or positive?
16 August 2022 7,969 5 View
was reading an older textbook (Duddeck) they describe H,C correlated COSY, interpreted as C connected to H. Is this essentially interpreted the same as HSQC and HETCOR?
10 July 2022 8,159 3 View
If I have extracted a plant with ethyl acetate and found that this extract has an effective activity, is it generally safe for humans to ingest this extracted plant material? Ethyl acetate is GRAS...
06 April 2021 550 6 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...
11 July 2024 4,829 0 View
The question refer to leafy biomass decomposition using litterbag techniques.
11 July 2024 2,078 0 View
When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?
07 July 2024 5,480 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View