I want to study insulin like growth factor from cervical fluid. After taken from cervical canal, sample will be diluted in pbs solution. My preliminary study shows unsatisfactory result. Should I add bsa instead?
I also find difficulties to store it in ultra low temperature due to for some hospital dont have those facilities.
can i store it in room temperature? what minimum temperature should I have to store it?
My plan is to preserve it in etanol 70% then added with solid carbondioxide at the outside. Room temprature approx 30-32 degree Celcius
saliva has a very high proteolytic activity. so if you preserve in Room temperature, you will have a significant degradation of RNA, DNA and proteins. please read this paper https://www.ncbi.nlm.nih.gov/pubmed/21917601 . if you have any doubt please let me know, good luck
You can find useful information in this booklet: Especially in chapter 2
http://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314807262343/litdoc28988741_20161015020944.pdf
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