I wanted to induce bacterila infection to the mmice and we have alwaays performed it in normal conditions by restraining the mouse but would it make a difference when we give small dose of isoflurane and then inject via tail vein?
Pavan Diwan Singh Raghuvanshi
In both condition injection is possible
You have to customise restraining before injection without anaesthesia
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Stefaan Van Biesen
For your personal ease, you can use isoflurane. However, it is know that anesthetics have (mild) immunosupressive traits. The next question is ofcourse if this is relevant towards your research.
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Layth Alkattan
I think you can induce infection with out using anesthesia or you can use some anesthetic agents out of vein to induce the state of local ischemia
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