Manovriti Thakur

4 Questions 7 Answers 0 Followers

Questions related from Manovriti Thakur

Can I perform tail vein injection to a mouse after anesthesia or I should inject only when mice in normal conditions without anesthesia?

I wanted to induce bacterila infection to the mmice and we have alwaays performed it in normal conditions by restraining the mouse but would it make a difference when we give small dose of...

12 December 2018 5,469 3 View

Does anybody knows how to identify B-1 cells in Immunohistochemistry?

I wanted to stain for B-1 Cells in mouse peritoneal cryo samples. I dont know which antibodies should i use for the classification , B220 and CD5 are one which i thought i could use? Does anyone...

04 April 2016 4,882 3 View

Indirect ELISA Standardization? Or Does anybody knows about Fibrin degradation products assays using one antibody?

I am using an fibrinogen antibody to detect Fibrin degradation products in the plasma clot supernatent. This antibody detects all the fragments of fibrinogen. I am using indirect ELISA for this, I...

03 March 2016 6,262 2 View

Could anybody help me with Cryo sections Immunohistochemistry Problems, I am staining for proteins in mouse liver cryo sections?

I am having problems with staining and tissue after the cryo sections. I fixed mouse liver in 8 percent PFA and then after 8 hr incubation i removed PFA and stored the organ at -80. After 3-4...

03 March 2016 1,322 11 View