I have purified the protein and want to do its CD and Fluorescence spectrometry, but I am stuck in the process of Fluorescence.
Suggest me the possible answer.
Hello experts, i purified a protein and found dimer at SDS-PAGE gel. I used BME and/or DTT to remove it but it couldn't remove the dimer. Is there any other way to remove protein dimer? Thanks
05 June 2018 165 9 View
Hello all, I am a Master student and in my M.Tech project, I am working on Chikungunya virus. I am trying to purify it's envelope protein. i have used affinity chromatography (His tag, Ni-NTA,...
04 May 2018 6,236 6 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View
Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...
20 February 2021 1,291 3 View
I'm using blast furnace slag for stabilizing subgrade soil.
17 February 2021 7,333 3 View