Ankur Singh

3 Questions 10 Answers 0 Followers

Questions related from Ankur Singh

Is there any other way to remove dimer from protein without using DTT or BME?

Hello experts, i purified a protein and found dimer at SDS-PAGE gel. I used BME and/or DTT to remove it but it couldn't remove the dimer. Is there any other way to remove protein dimer? Thanks

06 June 2018 233 9 View

How to purify an envelope protein with single desired band?

Hello all, I am a Master student and in my M.Tech project, I am working on Chikungunya virus. I am trying to purify it's envelope protein. i have used affinity chromatography (His tag, Ni-NTA,...

05 May 2018 6,299 6 View

Can I perform fluorescence spectrometry with 4 Tyrosine residues but no Tryptophan residue in 64 amino acids long protein?

I have purified the protein and want to do its CD and Fluorescence spectrometry, but I am stuck in the process of Fluorescence. Suggest me the possible answer.

04 April 2018 4,642 0 View