I am looking to measure lung wet/dry weight ratio and do the Evan's blue permeability assay in neonatal rats treated with LPS (inflammatory injury) and drug treatment to determine the development/extent of pulmonary edema as well as any potential benefits with the treatment.

I am wondering if I can collect one lung for lung wet/dry weight ratio measurements and the other for Evan's blue quantification, or do these two experiments have to be done separately?

- I understand the lung processing for each of these experiments is different so I'm wondering if collecting one lung for each experiment is enough or if the Evan's blue interferes with the lung wet/dry weights (even if the Evan's blue dye has been given to all animals and is controlled?)

- The other consideration is that I usually do a 24-hour LPS exposure for measuring lung wet/dry weight ratios, whereas I believe lungs are typically collected 30 minutes to 1 hour after Evan's blue IV injection so I would think it may not be suitable to do these two experiments on the same animal? Has anyone tried a longer evan's blue exposure?

Any insights or experience with these experiments would be appreciated, I am looking to maximise the use of each set of animals.