I want to measure the flouroscent signal from Hoechst stained fixed cell by a plate reader. Do I have to read the plate with PBS in it? Or, I can dry the plate and then can measure it?
Hi, a quick question? I just shifted to 10ul from our regular 25ul PCR protocol. I used SYBR Green in BioRad CFX. During set up the program, I forgot change the reaction volume from 25 to 10? I...
09 October 2019 3,041 3 View
I am planning to measure DNA concentration of fixed mammalian cells on 6 well plates. I am using Hoescht for staining. Do I need to use any cell permeabiling agent before I stain them? Should I...
09 October 2018 4,141 3 View
I'm treating my CHO cells with 100uM H202 for positive control. cells are dissociated by 20mM EDTA in 1X PBS. Trying to do all procedure under low light. 1% Low melting agarose temperature was...
02 March 2018 4,382 14 View
About confusing term: Intron, exon and nonfunctional or non-coding regions of genome. Are introns and the nonfunctional regions are same?
08 September 2012 5,789 15 View
Hi, I'm very new in this field. Can anybody tell me where should I start..??? Anybody pls suggest me some paper, from where I can understand what types of work is going on in this field..... I...
10 November 2011 750 17 View
Hello everybody, I'm a new member of this group. I've recently come to known about the term 'Cassette PCR'. But I couldn't gather enough information about it. Can anybody help me...???
05 June 2011 8,181 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View