Hi all,
I have transformed E.coli and currently growing it overnight in LB broth. My plan is to check for the presence of plasmid by PCR the next morning and do miniprep. My question is can i keep my transformed E.coli in LB broth for another 48 hours in 4 degree since I have problem with my miniprep kit.
Thank you
Save a 50uL aliquot at 4C for PCR analysis.
For the remaining liquid culture, spin down, decant, and store the pellet at -20C for future plasmid prep.
Yes, you can store your transformed E. coli in the refrigerator at 4 degrees Celsius for a short period, such as overnight or up to a few days. However, for longer-term storage, it's advisable to use a -20 degrees Celsius or -80 degrees Celsius freezer.
However, If the plasmid of interest you want to check for contains an antibiotic resistance gene, you might consider the antibiotic included in the storage medium. This would also ensure the selection of E. coli harboring the plasmid. I hope this helps
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