8 Questions 9 Answers 0 Followers
Questions related from Nur Mohd Faizal
Hi all, Does anyone know how to check cell viability of a fresh tissue? I am planning to take a fresh tissue from patient and transport it to our lab using transport media. How do i check if the...
11 July 2025 5,628 0 View
I had just thawed an old batch of BCS (from 2018), which had previously been thawed (probably due to freezer malfunction) once in 2020. The BCS stock appeared cloudy upon thawing, which I...
03 July 2025 9,353 0 View
I am having problem growing my 3T3-J2 which had a change of morphology after few passages? The cells were fine a day after thawing and looked healthy but started to have morphology changes and not...
25 June 2025 6,559 3 View
Hi, I have recently thawed a 3T3-J2 cells from our stock. Initially the cells look like the top image and as I passaged, it became more elongated and "more" fibroblastic/ spindle-like compared to...
09 June 2025 6,452 6 View
Hi, my lab uses Biorad Trans-blot system and the protocol was set to 25V for 27 mins (for 1.0mm gel). All this while, the transfer works fine with Biorad thick blot filter paper, however recently...
22 February 2024 2,654 1 View
I am currently growing BICR31 cancer cells for conditioned media collection, and as I passaged it I have seen many bubbles/vacuoles - like structures. Cells were healthy during the first split...
06 January 2021 2,732 5 View
I am currently collecting conditioned medium, where once the cells have reached confluence I will replace the culture medium with new complete medium and incubate 24h before collecting. But I have...
14 August 2020 1,540 6 View
Hi all, I have transformed E.coli and currently growing it overnight in LB broth. My plan is to check for the presence of plasmid by PCR the next morning and do miniprep. My question is can i...
01 January 1970 2,846 3 View
