Nur Mohd Faizal

4 Questions 5 Answers 0 Followers

Questions related from Nur Mohd Faizal

Why my gel wrinkled after transfer?

Hi, my lab uses Biorad Trans-blot system and the protocol was set to 25V for 27 mins (for 1.0mm gel). All this while, the transfer works fine with Biorad thick blot filter paper, however recently...

22 February 2024 2,566 1 View

Why do I see many vacuoles on my cancer cells?

I am currently growing BICR31 cancer cells for conditioned media collection, and as I passaged it I have seen many bubbles/vacuoles - like structures. Cells were healthy during the first split...

06 January 2021 2,520 5 View

Hi, does anyone know the different of using complete media and serum-free media to make conditioned media?

I am currently collecting conditioned medium, where once the cells have reached confluence I will replace the culture medium with new complete medium and incubate 24h before collecting. But I have...

14 August 2020 1,483 6 View

Can I keep my transformed E.coli in 4 degree before performing miniprep experiment?

Hi all, I have transformed E.coli and currently growing it overnight in LB broth. My plan is to check for the presence of plasmid by PCR the next morning and do miniprep. My question is can i...

01 January 1970 2,812 3 View