I did a temperature gradient PCR where I got specific band at 68 degrees.The extension temperature is also 68 degrees. When I run the product on gel I got a less intense amplicon for 68 degrees compared to other products.Is this because of the same annealing and extension temperature.Can I increase the extension temperature because the Taq polymearse I am using is recommended by the company (NEB) to be used at 68 degrees extension?Kindly help me in this regard.

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