Risha Hazarika

3 Questions 1 Answers 0 Followers

Questions related from Risha Hazarika

Can annealing and extension temperature be same?

I did a temperature gradient PCR where I got specific band at 68 degrees.The extension temperature is also 68 degrees. When I run the product on gel I got a less intense amplicon for 68 degrees...

27 February 2020 9,198 1 View

Can I increase The extension tempertaure of my Taq polymerase (Quick-Load® Taq 2X Master Mix from NEB) ?

I did a temperature gradient PCR where I got specific band at 68 degrees.The extension temperature is also 68 degrees. When I run the product on gel I got a less intense amplicon for 68 degrees...

27 February 2020 3,050 1 View

Can I increase The extension tempertaure of my Taq polymerase (Quick-Load® Taq 2X Master Mix from NEB) ?

I did a temperature gradient PCR where I got specific band at 68 degrees.The extension temperature is also 68 degrees. When I run the product on gel I got a less intense amplicon for 68 degrees...

02 February 2020 8,577 1 View