Can I harvest a mammalian culture batch with fungal contamination? Provided culture has shown good expression of proteins?

More Mr. Utkalendu Suvendusekhar Samantaray's questions See All

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are those seeking solution part of the problem?

In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?

20 July 2024 4,020 0 View

Is there any formula which is used to calculate the value of capacitive contribution in the case of battery type electrode?

Dunn's equation should not be used in the case of a battery-type electrode, according to a paper published in the journal "Phys. Chem. Lett. 2020, 11, 9846–9849 (ref. attached)" that I recently...

12 May 2024 3,261 0 View

Does multivariate NeuralProphet need future regressor to predict the future ?

Hello everyone,I am currently working with the multivariate time series forecasting by using NeuralProphet to predict the price of product.I used 7 economic indicators as regressor in my...

22 March 2024 8,265 3 View

I would like to know that prophet time series forecasting is under the category of machine learning or neural network?

I would like to know that prophet time series model is under the category of neural network or machine learning or deep learning? I want to forecast the price of product depending on other...

19 February 2024 1,243 1 View

How to do Strain controlled cyclic triaxial testing using GDS ELDYN triaxial set up?

Dear RG users, I hope all of you are doing well. I just wanted to know how can I do dispacement controlled cyclic triaxial tests using ELDYN dynamic equipment. As you can see in the figure, there...

14 September 2023 2,217 0 View

How to find the contributions of electronic and vibrational?

generally polar basis set will provide the tensors (dipole moment,polarizability and hyperpolarizability). but how to find their contributions separately

24 June 2023 1,296 6 View

Why is cell conditioned medium concentrated after collection? For transplantation experiments, can it be used without concentrating it?

To study the effects of cell conditioned medium (CM) in animals in vivo, is it necessary to concentrate the medium after collection? I am not familiar with the preparation of the CM.

01 March 2023 695 3 View

Is there any model which is more suitable for contrastive learning except SimCLR and MOCOv2?

These two models take a lot of time to train with powerful GPUs and required a minimum 512 batch size for good model performance.

28 February 2023 1,654 1 View

How to calculate the depletion width (width of space charge layer) using Mott-Schottky analysis?

Width of depletion region which can be remarked using width of space charge layer (WSCL).

06 February 2023 2,621 0 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Does the delta function actually make sense to use on point particles?

The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.

31 July 2024 10,109 3 View

Given the organizational complexity of academic institutions does an internal institutional politics play significant role in an institution's growth?

There are few business activities more prone to a credibility gap than the way in which executives approach organizational life. A sense of disbelief occurs when managers purport to make decisions...

08 July 2024 1,323 2 View

What are these round particles in cell culture flask?

I'm working with HEI-OC1 cells of the inner ear. Recently I'm seeing these round particles in the flask (picture attached), but cells' morphology and growth seems normal. However, there are...

26 June 2024 8,717 7 View

List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

Is there any bioinformatic tool that can download batch DNA seq and translate them into multiple aa seqs?

Hi, everyone I would like to know if there is there any bioinformatic tool that can download batch DNA seq using imported NCBI accession numbers (upto 100 sequences) and translate them into...

19 June 2024 1,917 2 View

My EMSA Blot is not sharp and blurry after exposure with a Phosphorimager (Typhoon). What can I do to fix that?

I'm currently trying to do some EMSA using P32. The first two gels looked perfect, with sharp bands and no background. However, all the following ones looked like the blot below. I'm not doing...

18 June 2024 1,967 2 View

How to run Activated Sludge Model (ADM1) in Aquasim software?

I am trying to model a sequencing batch reactor using ASM1 in aquasim. I started from scratch create variables and processes but it doesn't work as I expected. How can I find the ASM1 package...

17 June 2024 4,466 1 View

Why is SHSY5Y getting killed by protective drug?

I have received passage 29 from our vendor (NCCS, Pune). The cells are slightly different from the previous batch, they form neurospheres, and on adding 12% instead of 10% they show 90% monolayer....

10 June 2024 4,790 0 View

ROS-Glo kit (Promega) measures high blank, negative and solvent control in spheroids, any advice?

We are trying to measure intracellular H2O2 in spheroids post exposure to nanozymes, that should generate ROS, using the ROS-Glo kit from Promega. However, we are getting strange results. The...

09 June 2024 2,754 0 View