Can I gavage mice with FITC-dextran *and* collect intestine tissue for IHC?

05 February 2019 1 8K Report

Hi guys,

I will be dosing mice with FITC-dextran and collect their plasma to run an assay to measure intestinal permeability - however if possible, would also like to collect their intestines as well so I can fix them and perform IHC later.

Does anyone know if the FITC-dextran will affect the quality of the tissue for IHC purposes? If the FITC-dextran means IHC will be affected I will probably just go ahead with just the permeability assay.

Thanks!

Lucila Babio

Hi Sarah,

Did you finally solve this issue? If so, I'm curious how it worked.

Thanks in advance.

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