Hi everyone.
I exposed some brain sections, which were treated with 3H-DAMGO, on an autoradiographic film, and as usual in this case, the exposure time was about 1 week.
Unfortunately, at the end I couldn't scan the films so I am wondering if I can expose the same slides to another film.
Tritium has a very low radioactivity but it should be pretty stable (I think, according to the half-life) so maybe after 1 week there is still going to be enough radioactivity so that I can expose the slides again, maybe for a longer time.
What do you think?
Thank you in advance.
Francesco Giannone
Yes! The half life of tritium is about 12 years so there is plenty of time for a second exposure. That is if you use "apposition technique". (If you have sections and film permanently fixed together there might be a possibility that your tritiated compund might be dissolved during the developing process.)
Thank you a lot for the answer!
Yes, I thought that tritium was sufficiently stable but I wasn't sure.
I exposed the slides again and within a week I will see.
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