Francesco Giannone

7 Questions 4 Answers 0 Followers

Questions related from Francesco Giannone

Does agarose embedding of a brain sample affect Ab penetration and clearing in iDISCO protocol?

I want to immunolabel and clear some rat brain samples, therefore I decided to use the iDISCO protocol. Since the samples after this procedure become very fragile and the samples I want to analyse...

11 November 2018 6,717 2 View

Do you have any source where I can study how to properly analyze in vitro receptor autoradiography images?

I'm currenlty analyzing receptor autoradiography images of human brain sections using MCID software. I'm having some trouble understanding how properly analyzing the images, for example: what...

15 October 2018 7,527 0 View

Do you have any source where I can study how to properly analyze in vitro receptor autoradiography images?

I'm currenlty analyzing receptor autoradiography images of human brain sections using MCID software. I'm having some trouble understanding how properly analyzing the images, for example: what...

10 October 2018 954 0 View

Can I expose Tritium marked brain sections twice on autoradiographic film?

Hi everyone. I exposed some brain sections, which were treated with 3H-DAMGO, on an autoradiographic film, and as usual in this case, the exposure time was about 1 week. Unfortunately, at the end...

22 August 2018 6,320 2 View

[35S]GTPgS binding assay on rat brain sections, how can I make it work?

Hi everyone. In the last month I've been trying to do the [35S]GTPgS binding assay on rat brain sections using DAMGO as an agonist, so the goal is to assess the activation of the Mu Opioid...

17 August 2018 9,518 2 View

[35S]GTPgS binding assay on rat brain sections, how can I make it work?

Hi everyone. In the last month I've been trying to do the [35S]GTPgS binding assay on rat brain sections using DAMGO as an agonist, so the goal is to assess the activation of the Mu Opioid...

08 August 2018 3,995 2 View

What's the right dilution for anti-TH Antibody to use in whole rat brain imaging?

Hi all! I have to immunolabel whole rat brains (and then clear them) for light sheet microscopy imaging and I will use, as primary antibody, anti-TH antibody AB1542 (Sheep) from Millipore. I can...

05 May 2018 3,721 4 View