I mean adding the liquid food directly to the wholes in the well agar diffusion method without any solvents
At least (or best) use a water solution or dispersion. Potential antimicrobial efficacy in food will not disperse.
You can use DMSO or other organic solvents but your data will be effectively meaaningless technical trivia.
@Asem M Abdelshafy, what do you mean by "Liquid Food"?
@Nelson Masota, such as juices
It's better to be transparent or homogeneous without particulate matter can be opaque also, viscosity is another important factor for the anti-microbial testings. As Phil says, at least an aqueous solution is needed!
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