Can I differentiate between biofilm production and flagellum through visualization of these structures in MEV ?

17 May 2024 0 2K Report

I'm studying a Purple non-sulfur bacteria (Rhodopseudomonas palustris). I performed a scanning electron microscopy, but I'm strugglying to characterize their morphology at SEM. May someone help me to interpretate the features of the isolated strain?

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I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

What could be plastic adherent cells from PBMCs maintained in High Glucose DMEM 15% FBS ?

I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...

26 June 2024 3,664 1 View

Hemodialysis technician professional profile ?

What is the hemodialysis technician profile and the relationship to hemodialysis nurse, have you got experience about this or have you a technician among your staff?

01 May 2024 3,635 0 View

How to use AI in architecture design process?

29 January 2024 1,792 4 View

Does anyone know if there is any protocol for measuring the concentration of bile salts without using the kit proposed by INFOGEST?

28 November 2023 3,334 0 View

What is the approximate concentration of bile salts after measuring with the kit proposed by INFOGEST?

28 November 2023 8,122 0 View

What is the limit pass of T24 and RT4 bladder cell lines?

Can anyone tell me what is the maximum pass of T24 and RT4 bladder cell lines? Thanks

10 October 2023 2,978 0 View

What is the doubling time of T24 and RT4 bladder cell lines?

Can someone confirm that the doubling time for the T24 cell line is 19 hours? This is the information provided to me by the commercial house where we have purchased. (Cell Lines Service) And for...

10 October 2023 2,412 1 View

How is cryopreservation T24 and RT4 bladder cell lines?

Could someone tell me the freezing medium you use to freeze the RT4 and T24 bladder cells? I found on Elabscience (www.elabscience.com) that for T4 it is: 60% basal medium+30% FBS+10% DMSO. For...

10 October 2023 9,474 0 View

Have you ever used the ROUT method to find the outliers of a single variable instead of those of a non-linear model?

This method for finding outliers when fitting data in a non-linear regression model is explained in the following article. Detecting Outliers When Fitting Data With Nonlinear Regressi......

26 March 2023 2,529 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View