May I be able to detect WNV and sequence it in vectors and reservoirs in which have been dead? In another hand, I want to know if the WNV RNA genome remains when the vector or bird dies for some days room temperature situation (for example 1 week). I have not kept the mosquitoes in freezer. Please explain me. Thanks.
We routinely detect WNV RNA from dead mosquitoes that have been collected over 1 week. I do not know of a study that specifically tested the decay rate of WNV-RNA in a dead mosquito but If you can work with short pieces (eg. Illumina) I think you should be able to create cDNA libraries and sequence the full genome.
This paper may be of some help.
http://www.ncbi.nlm.nih.gov/pubmed/11931239
Great! Thanks Mike! I was not aware of this study by Mike Turell and colleagues.
Yes, we do not have any problem to detect viral RNA in dry mosquitoes kept at room temperature for some weeks. Full genome sequencing works as well.
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology
- Virus