We have currently in the lab only human cytokines and I wonder if the homology is big enough to be used to stimulate mouse cells or whether it is necessary to order mouse cytokines for this purpose.
Hi Silvina,
Unfortunately, in a word the answer to your question is NO. Why? Indeed theoretically in mice, T cells will be educated by the mouse thymus using mouse cytokines, such as IL-15. However, in the humanized mouse in order to improve the development of human NK cell populations, it did not work out. Thus species-specific cytokines produced by these transferred human cells and their (differentiated) progenitors were able to affect only these human cells ...
Best wishes,
Ilya
Hello Ilya
Thank you for your answer. Maybe the question was too obvious and the best is of course to use species matched cytokines, but some people have told me they use human IL-2 to stimulate mouse cells for example, so I was wodering if someone tried with other cytokines. Anyway maybe it would not be ideal since the affinity to the receptor will not be high enough..
Hi Silvina
For the three different cytokines you asked about there are three different answers.
Human IL-12p70 is not cross reactive for mouse cells (but mouse IL-12p70 has some cross reactivity on human cells).
Human IL-15 is cross reactive on mouse cells and mouse IL-15 activates human cells but with a lower bioactivity.
IL-18 is supposed to be not cross reactive between human and mouse.ö
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