Many labs I've interacted with protect their CNO solutions from ambient white lights (e.g. those of a wetlab) with opaque paper or aluminum foil. I have not been able to find a publications that has conclusively shown CNO as being sensitive to light; some commercial sites (like hellobio) claim that it is stable in solution in ambient light an air.
Is this a genuine thing, or is it one of those experimental myths that started somewhere and spread? It's not a big hassle to do some procedures in the dark, but brain microinfusions are a bit more difficult that way.
Any help is appreciated, thank you.
Hi Maruicio,
The chemists at Hello Bio have extensively tested the stability of CNO and CNO dihydrochloride in various different conditions, including ambient light. Don't want to contravene any posting rules, so can't include the web page here, but I can send you a link to the results directly if you would like, or the results are published on our website (just search for CNO, and in the Biological Description the following guides are referenced:
- Clozapine N-Oxide (freebase) - A technical review on stability, solubility and use in the lab
- Stability of Water-Soluble DREADD ligands in Solution: A Technical Review
Hope that this is of help!
Sam
hi Samantha,
I have seen the info on HelloBio. Honestly it's the only information I've really been able to find. It seems that the HPLC data provides indicates that there really isn't loss of purity until about 72h, but the powder remains stable regardless. The N=1 is only a single sample per solution, correct?
Ideally I'd like to find a peer reviewed publication to reference in manuscripts.
Hi Mauricio,
You are correct in that the current stability data on our website for CNO and CNO diHcl is for an N of 1. We have done stability testing for our more recent water-soluble DREADD compounds (JHU37152 dihydrochloride, JHU37160 dihydrochloride, uPSEM792 hydrochloride, Deschloroclozapine dihydrochloride (DCZ) with an N of 5, and the data will appear on our website soon. We are in the process of adding more N numbers to CNO and CNO diHCl and carrying out some enhanced stability studies with a view to publication, and will let you know when that is available.
Hope this is of some help,
Sam
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