Can glycerol solution for making bacterial glycerol stocks be autoclaved twice or a few times ?

Edvan Arifsaputra Suherman @Edvan_Arifsaputra_Suherman

06 June 2018 0 8K Report

My lab mate gave me a 87% glycerol stock solution that had been autoclaved a year ago. I made a 60% glycerol solution from it and autoclaved the 60% glycerol solution. I am going to use it to make bacterial glycerol stocks, but I am worried that autoclaving glycerol solution twice would damage the glycerol and thus can't be used as cell protectant in -80 oC.

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