Edvan Arifsaputra Suherman

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Questions related from Edvan Arifsaputra Suherman

Can glycerol solution for making bacterial glycerol stocks be autoclaved twice or a few times ?

My lab mate gave me a 87% glycerol stock solution that had been autoclaved a year ago. I made a 60% glycerol solution from it and autoclaved the 60% glycerol solution. I am going to use it to make...

06 June 2018 8,362 0 View

Could thermophilic bacteria that have been preserved in glycerol solution at -80 C for a year loss their ability to grow at high temperature?

I have 5 thermophilic bacteria that had been preserved for a year in glycerol solution (15%) at -80 oC. I tried to grow the five isolates in LB broth, 50 oC, 150 rpm, but only 3 isolates grew...

08 August 2017 7,168 4 View