Does percent recovery increases exponentially or linearly with the spiking levels?

Hello everyone, I have come across a discussion (related to PAHs) where it is stated that as the level of spiking increases (from lower spiked concentration to higher spiked concentration), the...

02 March 2019 4,470 3 View

How much justifiable it is to normalize the original data obtained for a sample as per the recoveries obtained (through using a recovery factor)?

Hello everyone, I had read few research papers on PAHs. Most of these papers report recovery levels for PAHs, an important QA/QC parameter, and report the concentrations for these compounds...

02 March 2019 5,027 3 View

Reason for low recovery of PAHs?

Hi everyone, I have been extracting PAHs from different food matrix but I am constantly facing the problem of low recovery. Please suggest me what may be the probable reason for low recoveries...

09 October 2017 4,448 2 View

Which recovery type for PAHs determination is right using LC-MS?

Hello Everyone! I have read the papers regarding LC-MS detection of PAHs in food. The internal standards are recommended for recovery in samples with this analytical method. I wonder how the...

01 January 1970 788 4 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View