Can FlowJo´s cell cycle analysis identifiy cells with more than 4n?

03 July 2014 1 2K Report

I'm analyzing cells with a mitotic defect, resulting in cells with more than 4n. I clearly see them in the cell cycle analysis (FACS) in the Fl2A-Histogramm. Can FlowJo (or any other programm) automatically identify this population? Using the normal "CellCycle analysis", the programm identifiys only the "normal" G1, S and G2 peaks.

Michelle L Kovarik

I think ModFit by Verity might be able to do this, although I have never tried it for anything but normal diploid cell cycles. You can download a free trial of the software to check it out. http://www.vsh.com/products/mflt/

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