We work with murine model to study Plasmodium berghei NK65 infection, wich causes lung damage in C57BL/6 mice, but some times I don't see this fenotipe. I would like to use 4-aminobenzoic acid to improve the parasites growth , but I don't know if its work.
I have used this acid, but for me there was no difference in the results.
The only experience with this is some work with P. chabaudi. The main effect was that when we used pyrimethamine resistant parasites, they were definitely dependent on addition of 4-aminobenzoic acid (which was added to the drinking water for the mice) for a sustainable infection. With wild type parasites, this effect was not seen. My interpretation of that is that the mutation leading to resistance makes the folate production less efficient, and the addition will boost the folate synthesis enough to overcome this.
I have experience working with the effect of PABA or p-amino benzoic acid against murine species (i.e. Pv, Pvp, Pcc, Pbb) in vivo in mice. This acid suppressed the parasites! Reversal of the effect can be seen once PABA is added in the drinking water. In your case, the addition of the acid might enhance parasite growth? Leading to increased virulene? Not sure, can have a trial experiment. Thanks.
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