20 August 2012 1 7K Report

I mean, to obtain a doping profile of the junction.

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Culture fibroblasts with or without FGF2?

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Flow cytometry compensation with beads - is the matrix the problem?

I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...

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Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!

26 June 2024 9,093 4 View

How do you culture THP1 Leukemic Stem Cells?

Hi! I have to test a drug on the LSC fraction of THP1, but I'm not finding any specific media to select and culture them. Do you know anyone? Thank you in advance

15 May 2024 1,464 3 View

Methanesulfonic acid neutralisation?

Hello, I am performing esterification reactions between fatty acids and alcohols, in presence of methanesulfonic acid and H3PO2 as catalysts. At the end of the reaction, to reach the acid index I...

22 April 2024 2,063 5 View

Method DIN EN 14110 ?

I need to analyze the residual methanol from methyl esters. I am wondering if there is someone who has this method DIN EN 14110 and is willing to share it with me? Thank you in advance, Elena

22 April 2024 387 0 View

What is the last resort washing solution for a pepsin column that has a blockage?

Recently my pepsin column got some blockage, supposedly because of the precipitation of aggregated protein. Multiple washes with 0.1% FA or pepsin wash (1.5M GuHCl + AcN + FA 95.2/4/0.8), as well...

20 April 2024 3,135 5 View

Why heptane is integrated in GC chromatograms ?

I am facing an issue with how chromatograms are recorded. From time to time, on our chromatograms the peak assigned to heptane is integrated as well and this gives us wrong results. Sometimes the...

07 April 2024 1,726 4 View

How can I calculate p-value from post hoc test for Kruskal-Wallis multiple comparisons in SAS?

I need to perform non-parametric post hoc multiple comparisons. I found two interesting macros that implemented methodology about post hoc test for Kruskal Wallis test, but p-value was never...

07 April 2024 288 3 View

What is the recommended sample size for the validation of a standard questionnaire consisting of 40 items?

Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...

26 March 2024 1,660 11 View

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