For synthesis of short peptides that only contain 3-6 amino acids, it is easy to synthesize them by chemical reactions. However, is it possible that we can transform E.coli with plasmid that is designed to express specific DNA fragment that encode these 3-6 amino acids? How efficient is this method compared to chemical synthesis?
Due to the cyclic nature of the methods of peptide synthesis, the concentration of full-length peptide synthesized in a given reaction is inversely correlated with the length of the proposed peptide. If you need good quantities of 5-10 base peptide it is much easier and cheaper to synthesize this peptide by solid phase chemical synthesis in comparison with recombinant technologies. In addition, expression of some peptides can be toxic for the host and several peptides are very sensitive to proteases thus leading to low yields. However, this problem could be solved by fusing the desired peptide to a carrier protein, but it also cost extra money.
yes but you have to express peptide with a big leader sequence and then purify the peptide.
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