Can DNase treatment be repeated on the same RNA sample which has been already treated with DNase?

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I want to simulate a system containing protein and heavy metals; which force field is most reliable for this purpose?

The protein I want to simulate has multiple heavy metals. I wanted to know which force-field I should use with GROMACS to achieve this. Most importantly, do the latest updates in the force-field...

16 February 2021 2,420 1 View

Under what conditions absorption and excitation spectra of a same species can be different?

I took absorption and excitation spectra of a same species in the same solvent. Ideally I should get the excitation spectra almost similar to the absorbance spectra. But I got the excitation...

13 December 2020 8,965 2 View

What are the good qualities of a teacher in current pandemic situation?

During the COVID-19 pandemic when all the teaching Institutions using online teaching methodologies. What are the qualities expected from the teacher?

12 July 2020 4,626 33 View

How do I separate cyclohexanol impurity?

I am doing a hydroboration-protonation reaction using dicyclohexylborane to reduce a triple bond to a cis-double bond. I use acetic acid, followed by sodium hydroxide and hydrogen peroxide in my...

02 May 2020 1,039 3 View

Measurement of Gain of a CP antenna?

How do I measure the gain of CP antenna inside an anachoic chamber? What should be the reference antenna, I mean it should be LP or CP? What is the procedure of measurement?

17 November 2019 336 6 View

How to obtain the sputtering yield of different species in Quaternary sputtering target?

we have prepared the CZTS thin films using single target in RF sputtering. which formula should i use to obtain the sputtering yield of different atoms such as Cu, Sn, Zn . is it possible to get...

15 October 2019 1,699 6 View

How to extend conference paper into journal paper?

I would like to know , what are the possible ways to extend conference paper into journal paper? My work is in the area of natural language processing

22 September 2019 1,246 3 View

How to obtain the crystallinity and strain in the thin film using RAMAN shift?

we have deposited the semiconducting thin film (CZTS) using sputtering. is it possible to obtain the crystallanity and strain in thin film using RAMAN spectra? which formula should i use for that?

09 September 2019 8,088 1 View

Is it possible to get different RAMAN shifts for the same sample deposited by different process parameters?

I have deposited CZTS by RF sputtering at different temperatures, pressures and powers. In RAMAN analysis i am getting peaks at 334,337,330 cm-1 is it possible to observe these kind of shifts due...

04 September 2019 5,898 2 View

What is miniaturization of Antenna?

How we will calculate the miniaturization of antenna? What are the parameters are to be consider to calculate the miniaturization? Is there any proper formula?

13 August 2019 7,475 6 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View