We have performed the denaturation in FISH with normal formamide (73 C), but it seems that the probes did not attach to the chromosomes, there are only non-specific signals. I wonder whether it could be due to that formamide was not deionised?
http://www.ucl.ac.uk/~ucbzwdr/In%20Situ%20Protocol.pdf
I have no personal experience with your question, but was curious about it. I found the information above. Maybe it helps.
The author of this text has the opinion that the difference matters.
You can do denaturation with normal formamide. I recommend keeping the formamide at -20C for long storage. Stocks solutions of denaturation solution can be made several weeks in advance. These should be stored at 4C until,
Thank you for your answers and thanks for the link, it seems very useful!
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